Journal: bioRxiv

Article Title: Probing the functions of microglial cyclin-dependent kinase 5 under physiological and pathological conditions

doi: 10.1101/2020.05.12.090605

Figure Lengend Snippet: (A and B) Graphs showing total distance travelled (A) and percent time the mice spent in the center (B) in the open field test. Unpaired t-tests were used, n=6 mice in control and n=8 mice in Cdk5cKO mice. (C and D) Graphs showing percent time in light chamber (C) and latency to light chamber (D) in the light dark tests. Unpaired t-tests were used, n=6 mice in control and n=8 mice in Cdk5cKO mice. (E and F) Graphs showing discrimination index (E) and novel object exploration time (F) in the novel object recognition test. Unpaired t-tests were used, n=6 mice in control and n=8 mice in Cdk5cKO mice. (G and H) Images (G) and quantification (H) of synaptophysin signal intensity in the CA1 of the hippocampus. Unpaired t-tests were used, n=6 mice in control and n=7 mice in Cdk5cKO mice. (I and J) Images (I) and quantification (J) of vGAT puncta in the CA1 of the hippocampus. Unpaired t-tests were used, n=6 mice in control and n=7 mice in Cdk5 cKO mice.

Article Snippet: Primary antibodies used in this study include anti-β-amyloid (D54D2)(1:500; Cell Signaling Technology; 8243), anti-Iba1 (1:500; Synaptic Systems 234 004, polyclonal guinea pig anti-serum), anti-Synaptophysin antibody (1:500; Abcam ab32137) and VGAT (1:500; Synaptic Systems 131 013).

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